The Breeder Blog

Relaxin Pregnancy Test for Dogs: A Reliable Tool for Breeding Success

by Phenyx Media on Jul 08, 2023

Relaxin Pregnancy Test for Dogs: A Reliable Tool for Breeding Success

Introduction to Relaxin Pregnancy Test for Dogs

As a dog breeder or a proud pet parent, there are few things as nerve-wracking and yet exciting as anticipating a litter of puppies. The wait can be filled with uncertainty, and naturally, you want answers as soon as possible. This is where the Relaxin Pregnancy Test for dogs comes into play. This article will walk you through everything you need to know about this test - from what it is, how it works, to how it can help ensure breeding success.

Understanding Relaxin: The Pregnancy Hormone in Dogs

Before we delve into the test itself, let's first discuss the star of the show: relaxin. It's not a magical potion, but for dog breeders, it can be just as potent. Relaxin is a hormone that female dogs produce when they're pregnant. It's primarily generated within the developing placental tissues. It plays a significant role in preparing the uterus for pregnancy and facilitates the growth and development of the puppies.

How Relaxin Pregnancy Test Works

At its core, the Relaxin Pregnancy Test for dogs is designed to detect the presence of the relaxin hormone in a dog's blood. The test uses specialized antibodies that bind to relaxin if present. This reaction triggers a visible signal on the test, indicating pregnancy.

Advantages of Relaxin Pregnancy Test

Why choose the Relaxin Pregnancy Test over other methods? First, it's reliable. While other tests or observations might provide ambiguous results, the Relaxin Pregnancy Test is definitive. Second, it's safe. Since the procedure is non-invasive, it doesn't put the mother or her puppies at risk. Lastly, it's fast and straightforward. The entire process can be completed within a couple of hours, and you don't need extensive lab equipment or training to conduct the test.

When to Conduct a Relaxin Pregnancy Test

relaxin dog pregnancy test kit sitting on a wood table

Knowing when to conduct the test is crucial. The timing can significantly impact the accuracy of the results.

Best Timing for the Test

The ideal time to conduct the Relaxin Pregnancy Test is approximately 21-28 days post-breeding. Why this specific timeframe? At around three to four weeks post-breeding, the relaxin hormone level in the dog's blood is usually sufficient to be detected by the test.

Signs to Look Out For

While timing is essential, it's also helpful to keep an eye out for signs that indicate pregnancy in dogs. These signs can vary from dog to dog, but some common indicators include changes in appetite, swollen nipples, increased affection or nesting behavior, and a decrease in energy levels. If you observe these signs alongside the appropriate timing, it may be a good indication to proceed with the Relaxin Pregnancy Test.

How to Administer a Relaxin Pregnancy Test

Now that you understand the importance of the test and when to conduct it, let's explore how to administer the Relaxin Pregnancy Test.

The Collection Process

Collecting a blood sample from your dog is the first step. It's recommended to have a veterinarian perform this procedure to ensure it is done correctly and safely. The vet will typically draw a small amount of blood from a vein in the dog's leg or neck. The process is relatively quick and minimally invasive.

The Testing Procedure

Once the blood sample is collected, it's time to perform the test. The Relaxin Pregnancy Test kit usually contains all the necessary components, including test strips and instructions. Following the instructions provided, you'll add the blood sample to the designated area on the test strip. The strip will then show the presence or absence of the relaxin hormone, indicating whether the dog is pregnant or not.

Interpreting the Results of a Relaxin Pregnancy Test

Two golden puppies sitting on grass

After conducting the test, it's essential to understand how to interpret the results accurately.

Positive Results

A positive result means that relaxin was detected in the blood sample. This indicates that the dog is pregnant. It's an exciting confirmation that your breeding efforts have been successful, and you can start planning for the arrival of the puppies.

Negative Results

A negative result indicates that relaxin was not detected in the blood sample. This means that the dog is not pregnant. However, it's important to note that false negatives can occur, especially if the test is conducted too early in the pregnancy. If you strongly suspect pregnancy despite a negative result, it's advisable to consult with your veterinarian for further evaluation.

Importance of Relaxin Pregnancy Test in Breeding Success

Now that we've covered the ins and outs of the Relaxin Pregnancy Test, let's explore why it is a crucial tool for breeding success.

Planning the Breeding Program

Breeding dogs requires careful planning and timing. By using the Relaxin Pregnancy Test, breeders can accurately determine if a mating has resulted in pregnancy. This knowledge allows them to move forward with confidence in their breeding program, selecting the appropriate stud, and making informed decisions about future pairings.

Ensuring Health and Safety of the Bitch

Pregnancy can pose risks and challenges for the mother dog. By confirming pregnancy through the Relaxin Pregnancy Test, breeders can ensure the health and well-being of the pregnant bitch. They can provide proper prenatal care, adjust the diet accordingly, and monitor for any potential complications that may arise during the gestation period.


In conclusion, the Relaxin Pregnancy Test for dogs is a valuable tool that provides breeders and pet owners with a reliable and efficient method of confirming canine pregnancy. By understanding the timing, signs to look out for, and the testing process, breeders can enhance their breeding programs and ensure the health and safety of the pregnant dogs. Incorporating the Relaxin Pregnancy Test into your breeding journey can bring peace of mind and set the stage for a successful and joyful experience.

Frequently Asked Questions About Relaxin Pregnancy Tests

Q: How early can I conduct the Relaxin Pregnancy Test after breeding?

A: The test is typically reliable starting from 21-28 days post-breeding.

Q: Can the Relaxin Pregnancy Test give false results?

A: While rare, false negatives can occur, especially if the test is conducted too early. It's recommended to consult with a veterinarian if you suspect pregnancy despite a negative result.

Q: Can I perform the Relaxin Pregnancy Test at home?

A: The test can be conducted at home using a test kit, but it's advisable to have a veterinarian collect the blood sample to ensure accuracy and safety.

Q: Are there any risks involved in the Relaxin Pregnancy Test?

A: The test is non-invasive and generally safe for dogs. However, it's always best to have a professional handle the blood collection process.

Q: Can male dogs produce relaxin hormone?

A: No, relaxin is primarily produced by pregnant female dogs and is not present in males.